The focus of the work will be on evaluating the magnitude of the fluorescence shifts and enhancement of plasmon-coupled fluorophores using single molecule localization microscopy and time-resolved single molecule spectroscopy. Single molecule localization microscopy overcomes the diffraction limit by calculating the center positions of a fluorescent spot based on the known point spread function of the optical microscope. While plasmonic coupling is known to strongly increase the number of emitted photons from a fluorophore, it also appears to affect the position of the emission. The work will consist of visualization of assembled fluorophores with and withoutz plasmonic nanoparticles, data analysis and statistics.
Funding category: Contrat doctoral
PHD title: PhD
PHD Country: Tchèque, République
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