Post-doctoral fellowship for high resolution electrophysiology of human brain organoids(M/F)

Updated: 4 months ago
Job Type: FullTime
Deadline: 04 Aug 2021

The prospective postdoctoral researcher should understand the concept of the neuronal activity and circuit formation, and learn experimental details such as cell culture, microfabrication, electrophysiological and immunohistochemical characterization. Then, he or she is supposed to start a more systematic work on optimization and characterization of Nano Electrode Arrays for analysis of brain organoids' activity. Furthermore, measurements and analysis of macroscopic circuit models generated by connecting brain organoids will be attempted. The candidate will search for characteristic patterns of neuronal activity produced by particular circuit configuration e.g. organoid types and connection geometry.

Organoid technologies have attracted increasing attention due to their unprecedented potential for modeling human organs in vitro by mimicking differentiation and self-organization in vivo. Brain organoids can help us overcome major issues of neuroscience research, including the limited access to viable human primary tissue, the difficulties of investigating human brains non-invasively, and the poor match of animal models of neurological diseases with the human pathophysiology. One limitation of the current development of brain organoid research is the lack of technological tool to monitor electrophysiological activity for a long period with high resolution and sensitivity. By using a Nano Electrode Arrays technology [1] developed at LAAS-CNRS in Toulouse, we demonstrated at Ikeuchi Lab (Institute of Industrial Science, Tokyo), high resolution interfacing of organoids thanks to the high affinity of the nanoprobes with the cells, leading to unpreceded electrophysiology details on such in vitro 3D tissues. The Ikeuchi Lab is developing methods to generate macroscopic circuits of human neurons by connecting organoids through axon bundles in a microfluidic chip, mimicking the physical environment in custom-made microchips [2]. With this original strategy and the state-of-the-art technologies, our team is poised to understand brain circuits and related diseases.
We are looking for a postdoc researcher to join our project to further pursue these developments at Ikeuchi lab with emphases on the construction, integration and thorough investigation of connected organoids from different regions of the brain using the nanoelectrode array platform, in collaboration with LAAS-CNRS.

[1] A. Casanova et al. J. Phys. Cond. Mat. 30 (2018), A. Casanova et al. IEEE IEDM 2017, 26.4.
[2] T. Osaki and Y. Ikeuchi. BioRxiv (2021), T. Kirihara et al. iScience (2019), J. Kawada et al. Stem Cell Reports (2017)

LIMMS/CNRS-IIS UMI 2820 (Laboratory for Integrated Micro Mechatronic Systems) is an international laboratory between the French CNRS (Centre National de la Recherche Scientifique) and Institute of Industrial Science (IIS), The University of Tokyo, located in Komaba, Tokyo. LIMMS has more than 24 years of experience in international cooperative research and has welcomed more than 240 researchers from France and Europe.
LIMMS opens a new postdoctoral position in the laboratory of Professor Yoshiho Ikeuchi, Institute of Industrial Science, University of Tokyo, collaboration with Guilhem Larrieu, LIMMS/CNRS-IIS (UMI 2820). . The laboratory is well equipped with apparatus for tissue culture, imaging, and electrophysiological analyses. The lab is interdisciplinary, international and diverse.

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