Research Lab Technician II

Updated: 2 months ago
Location: Los Angeles, CALIFORNIA

Our laboratory in the Department of Pathology and Laboratory Medicine located at USC Keck School of Medicine is seeking a full-time Research Laboratory Technician II to join our research team of highly skilled senior research associates or technicians, and junior faculty researchers. Under the direction of Principle Investigators, our research studies focus on peptidic effectors of mammalian innate immunity and for drug development.

Our laboratory employs an array of general scientific laboratory research practices and procedures including molecular biology, protein biochemistry, enzyme kinetic, immune signaling pathways, microbiology; and animal models for acute and chronic inflammatory diseases. Under direct supervision, he/she will be performing peptide drug susceptibility testing in rodent animal models following Institutional Animal Care and Use Committee approved protocols. He/she will be testing a library of synthetic peptides for their antimicrobial or immunomodulatory activities against  panels of multidrug resistant bacteria (e.g. Klebsiella pneumoniae ), or fungal pathogens (e.g. Candida albicans ); and or for their efficacy in  immune-related pathologies in rodents such as  tumor growth, colitis or  rheumatoid arthritis. He/she will employ sterile tissue culture techniques to grow and maintain, and use multi drug resistant organisms for infections under approved biosafety level 2 protocols.  

The incumbent will administer peptide treatment while collecting data for clinical/in-life presentation of disease (i.e. survivability, weight gain/loss, disease severity, mortality, and/or morbidity), post-mortem indicators of disease (i.e. bacterial or fungal burden).  He/she will be responsible for obtaining, maintaining, and graphing of data. Technician will inoculate rodents with pathogens by intraperitoneal or intravenous injections and he/she will give drug treatment or placebos to infected animals, and harvest organs for histochemical analysis; collect blood for pharmacokinetics , and enumerate bacterial/fungal load as a function of peptide treatment. In our inflammatory disease models, maintaining a rodent colony, measuring weights and recording visual observations may be employed. Additional pharmacokinetics will be analyzed utilizing uninfected rabbits as a secondary model. Additional peptide libraries may be  generated by recombinant expression. Candidate will need to complete animal tutorial training and receive required certification in general laboratory safety prior to any work performed in the laboratory.

Shift: Mon-Fri ^8:30am - 5pm (Flexible hours will be required, some weekends)

Previous general scientific laboratory research practices and rodent animal handling is required for this position.

This is Grant Funded position

The University offers a competitive salary and benefits package.

Salary: DOE

Minimum Education: Associate's degree, Specialized/technical training, Combined experience/education as substitute for minimum education Minimum Experience: 2 years Minimum Field of Expertise: Working knowledge of laboratory equipment and research protocols. Knowledge of computer analysis techniques and ability to provide routine analysis and interpretation of research data.

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